1: Smaldone G, Diana D, Pollegioni L, Di Gaetano S, Fattorusso R, Pedone E.
Insight into conformational modification of alpha-synuclein in the presence of neuronal whole cells and of their isolated membranes. FEBS Lett. 2015 Feb 18.
pii: S0014-5793(15)00092-7. doi: 10.1016/j.febslet.2015.02.012. [Epub ahead of print] PubMed PMID: 25701590.

2: Cappelletti P, Piubelli L, Murtas G, Caldinelli L, Valentino M, Molla G, Pollegioni L, Sacchi S. Structure-function relationships in human d-amino acid oxidase variants corresponding to known SNPs. Biochim Biophys Acta. 2015 Feb 17.
pii: S1570-9639(15)00041-2. doi: 10.1016/j.bbapap.2015.02.005. [Epub ahead of print] PubMed PMID: 25701391.

3: Pollegioni L, Tonin F, Rosini E. Lignin-degrading enzymes. FEBS J. 2015 Feb 3.
doi: 10.1111/febs.13224. [Epub ahead of print] PubMed PMID: 25649492.

4: Le Bail M, Martineau M, Sacchi S, Yatsenko N, Radzishevsky I, Conrod S, Ait Ouares K, Wolosker H, Pollegioni L, Billard JM, Mothet JP. Identity of the NMDA receptor coagonist is synapse specific and developmentally regulated in the
hippocampus. Proc Natl Acad Sci U S A. 2015 Jan 13;112(2):E204-13. doi:
10.1073/pnas.1416668112. Epub 2014 Dec 30. PubMed PMID: 25550512; PubMed Central PMCID: PMC4299231.

5: Temporini C, Bavaro T, Tengattini S, Serra I, Marrubini G, Calleri E, Fasanella F, Piubelli L, Marinelli F, Pollegioni L, Speranza G, Massolini G, Terreni M. Liquid chromatography-mass spectrometry structural characterization of neo glycoproteins aiding the rational design and synthesis of a novel glycovaccine for protection against tuberculosis. J Chromatogr A. 2014 Nov
7;1367:57-67. doi: 10.1016/j.chroma.2014.09.041. Epub 2014 Sep 22. PubMed PMID: 25282312.

6: Molla G, Nardini M, Motta P, D'Arrigo P, Panzeri W, Pollegioni L. Aminoacetone oxidase from Streptococcus oligofermentans belongs to a new three-domain family of bacterial flavoproteins. Biochem J. 2014 Dec 15;464(3):387-99. doi:
10.1042/BJ20140972. PubMed PMID: 25269103.

7: Labarrière M, Thomas F, Dutar P, Pollegioni L, Wolosker H, Billard JM. Circuit-specific changes in D-serine-dependent activation of the N-methyl-D-aspartate receptor in the aging hippocampus. Age (Dordr).
2014;36(5):9698. doi: 10.1007/s11357-014-9698-0. Epub 2014 Aug 20. PubMed PMID: 25138794.

8: Terry-Lorenzo RT, Chun LE, Brown SP, Heffernan ML, Fang QK, Orsini MA, Pollegioni L, Hardy LW, Spear KL, Large TH. Novel human D-amino acid oxidase inhibitors stabilize an active-site lid-open conformation. Biosci Rep. 2014 Aug
11;34(4). pii: e00133. doi: 10.1042/BSR20140071. PubMed PMID: 25001371; PubMed Central PMCID: PMC4127593.

9: Rosini E, Piubelli L, Molla G, Frattini L, Valentino M, Varriale A, D'Auria S, Pollegioni L. Novel biosensors based on optimized glycine oxidase. FEBS J. 2014 Aug;281(15):3460-72. doi: 10.1111/febs.12873. Epub 2014 Jul 1. PubMed PMID:

10: Nicolia A, Ferradini N, Molla G, Biagetti E, Pollegioni L, Veronesi F, Rosellini D. Expression of an evolved engineered variant of a bacterial glycine oxidase leads to glyphosate resistance in alfalfa. J Biotechnol. 2014 Aug
20;184:201-8. doi: 10.1016/j.jbiotec.2014.05.020. Epub 2014 Jun 4. PubMed PMID: 24905148.

11: Canu N, Ciotti MT, Pollegioni L. Serine racemase: a key player in apoptosis and necrosis. Front Synaptic Neurosci. 2014 Apr 21;6:9. doi:10.3389/fnsyn.2014.00009. eCollection 2014. Review. PubMed PMID: 24795622; PubMed
Central PMCID: PMC4000995.

12: Lench AM, Massey PV, Pollegioni L, Woodhall GL, Jones RS. Astroglial d-serine is the endogenous co-agonist at the presynaptic NMDA receptor in rat entorhinal
cortex. Neuropharmacology. 2014 Aug;83:118-27. doi:
10.1016/j.neuropharm.2014.04.004. Epub 2014 Apr 18. PubMed PMID: 24747728.

13: Conti G, Pollegioni L, Molla G, Rosini E. Strategic manipulation of an industrial biocatalyst--evolution of a cephalosporin C acylase. FEBS J. 2014 May;281(10):2443-55. doi: 10.1111/febs.12798. Epub 2014 Apr 22. PubMed PMID:

14: Polcari D, Kwan A, Van Horn MR, Danis L, Pollegioni L, Ruthazer ES, Mauzeroll J. Disk-shaped amperometric enzymatic biosensor for in vivo detection of D-serine. Anal Chem. 2014 Apr 1;86(7):3501-7. doi: 10.1021/ac404111u. Epub 2014
Mar 20. PubMed PMID: 24650010.

15: Piubelli L, Campa M, Temporini C, Binda E, Mangione F, Amicosante M, Terreni
M, Marinelli F, Pollegioni L. Optimizing Escherichia coli as a protein expression
platform to produce Mycobacterium tuberculosis immunogenic proteins. Microb Cell
Fact. 2013 Nov 19;12:115. doi: 10.1186/1475-2859-12-115. PubMed PMID: 24252280;
PubMed Central PMCID: PMC4225511.

16: Cappelletti P, Campomenosi P, Pollegioni L, Sacchi S. The degradation (by
distinct pathways) of human D-amino acid oxidase and its interacting partner
pLG72--two key proteins in D-serine catabolism in the brain. FEBS J. 2014
Feb;281(3):708-23. doi: 10.1111/febs.12616. Epub 2013 Dec 10. PubMed PMID:

17: Pollegioni L, Motta P, Molla G. L-amino acid oxidase as biocatalyst: a dream
too far? Appl Microbiol Biotechnol. 2013 Nov;97(21):9323-41. Review. PubMed PMID:

18: Rosini E, Tonin F, Vasylieva N, Marinesco S, Pollegioni L. Evolution of
histamine oxidase activity for biotechnological applications. Appl Microbiol
Biotechnol. 2014 Jan;98(2):739-48. doi: 10.1007/s00253-013-5159-4. Epub 2013 Aug
31. PubMed PMID: 23995223.

19: Raimondi I, Ciribilli Y, Monti P, Bisio A, Pollegioni L, Fronza G, Inga A,
Campomenosi P. P53 family members modulate the expression of PRODH, but not
PRODH2, via intronic p53 response elements. PLoS One. 2013 Jul 8;8(7):e69152.
doi: 10.1371/journal.pone.0069152. Print 2013. PubMed PMID: 23861960; PubMed
Central PMCID: PMC3704516.

20: Hopkins SC, Heffernan ML, Saraswat LD, Bowen CA, Melnick L, Hardy LW, Orsini
MA, Allen MS, Koch P, Spear KL, Foglesong RJ, Soukri M, Chytil M, Fang QK, Jones
SW, Varney MA, Panatier A, Oliet SH, Pollegioni L, Piubelli L, Molla G, Nardini
M, Large TH. Structural, kinetic, and pharmacodynamic mechanisms of D-amino acid
oxidase inhibition by small molecules. J Med Chem. 2013 May 9;56(9):3710-24. doi:
10.1021/jm4002583. Epub 2013 Apr 30. PubMed PMID: 23631755.

21: Mattioni C, Martin MA, Pollegioni P, Cherubini M, Villani F. Microsatellite
markers reveal a strong geographical structure in European populations of
Castanea sativa (Fagaceae): evidence for multiple glacial refugia. Am J Bot. 2013
May;100(5):951-61. doi: 10.3732/ajb.1200194. Epub 2013 Apr 23. PubMed PMID:

22: Li Y, Sacchi S, Pollegioni L, Basu AC, Coyle JT, Bolshakov VY. Identity of
endogenous NMDAR glycine site agonist in amygdala is determined by synaptic
activity level. Nat Commun. 2013;4:1760. doi: 10.1038/ncomms2779. PubMed PMID:
23612301; PubMed Central PMCID: PMC3641574.

23: Maucler C, Pernot P, Vasylieva N, Pollegioni L, Marinesco S. In vivo D-serine
hetero-exchange through alanine-serine-cysteine (ASC) transporters detected by
microelectrode biosensors. ACS Chem Neurosci. 2013 May 15;4(5):772-81. doi:
10.1021/cn4000549. Epub 2013 Apr 12. PubMed PMID: 23581544; PubMed Central PMCID:

24: Caldinelli L, Albani D, Pollegioni L. One single method to produce native and
Tat-fused recombinant human α-synuclein in Escherichia coli. BMC Biotechnol. 2013
Apr 4;13:32. doi: 10.1186/1472-6750-13-32. PubMed PMID: 23557146; PubMed Central
PMCID: PMC3621789.

25: Curcio L, Podda MV, Leone L, Piacentini R, Mastrodonato A, Cappelletti P,
Sacchi S, Pollegioni L, Grassi C, D'Ascenzo M. Reduced D-serine levels in the
nucleus accumbens of cocaine-treated rats hinder the induction of NMDA
receptor-dependent synaptic plasticity. Brain. 2013 Apr;136(Pt 4):1216-30. doi:
10.1093/brain/awt036. Epub 2013 Mar 20. PubMed PMID: 23518710.

26: Binda E, Marcone GL, Berini F, Pollegioni L, Marinelli F. Streptomyces spp.
as efficient expression system for a D,D-peptidase/D,D-carboxypeptidase involved
in glycopeptide antibiotic resistance. BMC Biotechnol. 2013 Mar 16;13:24. doi:
10.1186/1472-6750-13-24. PubMed PMID: 23497129; PubMed Central PMCID: PMC3610138.

27: Pollegioni L, Rosini E, Molla G. Cephalosporin C acylase: dream and(/or)
reality. Appl Microbiol Biotechnol. 2013 Mar;97(6):2341-55. doi:
10.1007/s00253-013-4741-0. Epub 2013 Feb 16. Review. PubMed PMID: 23417342.

28: Bava A, Gornati R, Cappellini F, Caldinelli L, Pollegioni L, Bernardini G.
D-amino acid oxidase-nanoparticle system: a potential novel approach for cancer
enzymatic therapy. Nanomedicine (Lond). 2013 Nov;8(11):1797-806. doi:
10.2217/nnm.12.187. Epub 2013 Feb 5. PubMed PMID: 23384700.

29: Golden E, Paterson R, Tie WJ, Anandan A, Flematti G, Molla G, Rosini E,
Pollegioni L, Vrielink A. Structure of a class III engineered cephalosporin
acylase: comparisons with class I acylase and implications for differences in
substrate specificity and catalytic activity. Biochem J. 2013 Apr
15;451(2):217-26. doi: 10.1042/BJ20121715. PubMed PMID: 23373797.

30: Bifulco D, Pollegioni L, Tessaro D, Servi S, Molla G. A thermostable
L-aspartate oxidase: a new tool for biotechnological applications. Appl Microbiol
Biotechnol. 2013 Aug;97(16):7285-95. doi: 10.1007/s00253-013-4688-1. Epub 2013
Jan 31. PubMed PMID: 23371294.

31: Caldinelli L, Sacchi S, Molla G, Nardini M, Pollegioni L. Characterization of
human DAAO variants potentially related to an increased risk of schizophrenia.
Biochim Biophys Acta. 2013 Mar;1832(3):400-10. doi: 10.1016/j.bbadis.2012.11.019.
Epub 2012 Dec 5. PubMed PMID: 23219954.

32: Sacchi S, Rosini E, Pollegioni L, Molla G. D-amino acid oxidase inhibitors as
a novel class of drugs for schizophrenia therapy. Curr Pharm Des.
2013;19(14):2499-511. Review. PubMed PMID: 23116391.

33: Sacchi S, Caldinelli L, Cappelletti P, Pollegioni L, Molla G.
Structure-function relationships in human D-amino acid oxidase. Amino Acids. 2012
Nov;43(5):1833-50. doi: 10.1007/s00726-012-1345-4. Epub 2012 Aug 3. Review.
PubMed PMID: 22865246.

34: Papouin T, Ladépêche L, Ruel J, Sacchi S, Labasque M, Hanini M, Groc L,
Pollegioni L, Mothet JP, Oliet SH. Synaptic and extrasynaptic NMDA receptors are
gated by different endogenous coagonists. Cell. 2012 Aug 3;150(3):633-46. doi:
10.1016/j.cell.2012.06.029. PubMed PMID: 22863013.

35: Binda E, Marcone GL, Pollegioni L, Marinelli F. Characterization of VanYn, a
novel D,D-peptidase/D,D-carboxypeptidase involved in glycopeptide antibiotic
resistance in Nonomuraea sp. ATCC 39727. FEBS J. 2012 Sep;279(17):3203-13. doi:
10.1111/j.1742-4658.2012.08706.x. Epub 2012 Aug 2. PubMed PMID: 22788848.

36: Esposito S, Pristerà A, Maresca G, Cavallaro S, Felsani A, Florenzano F,
Manni L, Ciotti MT, Pollegioni L, Borsello T, Canu N. Contribution of serine
racemase/d-serine pathway to neuronal apoptosis. Aging Cell. 2012
Aug;11(4):588-98. doi: 10.1111/j.1474-9726.2012.00822.x. Epub 2012 Jun 5. PubMed
PMID: 22507034.

37: Pernot P, Maucler C, Tholance Y, Vasylieva N, Debilly G, Pollegioni L,
Cespuglio R, Marinesco S. d-Serine diffusion through the blood-brain barrier:
effect on d-serine compartmentalization and storage. Neurochem Int. 2012
Jun;60(8):837-45. doi: 10.1016/j.neuint.2012.03.008. Epub 2012 Mar 23. PubMed
PMID: 22465696.

38: Tallarita E, Pollegioni L, Servi S, Molla G. Expression in Escherichia coli
of the catalytic domain of human proline oxidase. Protein Expr Purif. 2012
Apr;82(2):345-51. doi: 10.1016/j.pep.2012.01.021. Epub 2012 Feb 8. PubMed PMID:

39: Volontè F, Pisanelli I, D'Arrigo P, Viani F, Molla G, Servi S, Pollegioni L.
Overexpression of a bacterial chymotrypsin: application for L-amino acid ester
hydrolysis. Enzyme Microb Technol. 2011 Dec 10;49(6-7):560-6. doi:
10.1016/j.enzmictec.2011.06.009. Epub 2011 Jun 16. PubMed PMID: 22142732.

40: Frattini LF, Piubelli L, Sacchi S, Molla G, Pollegioni L. Is rat an
appropriate animal model to study the involvement of D-serine catabolism in
schizophrenia? Insights from characterization of D-amino acid oxidase. FEBS J.
2011 Nov;278(22):4362-73. doi: 10.1111/j.1742-4658.2011.08354.x. Epub 2011 Oct
10. PubMed PMID: 21981077.

41: Tedeschi G, Pollegioni L, Negri A. Assays of D-amino acid oxidases. Methods
Mol Biol. 2012;794:381-95. doi: 10.1007/978-1-61779-331-8_26. PubMed PMID:

42: Sacchi S, Rosini E, Caldinelli L, Pollegioni L. Biosensors for D-amino acid
detection. Methods Mol Biol. 2012;794:313-24. doi: 10.1007/978-1-61779-331-8_21.
PubMed PMID: 21956573.

43: Ghisla S, Pollegioni L, Molla G. Revisitation of the βCl-elimination reaction
of D-amino acid oxidase: new interpretation of the reaction that sparked
flavoprotein dehydrogenation mechanisms. J Biol Chem. 2011 Nov
25;286(47):40987-98. doi: 10.1074/jbc.M111.266536. Epub 2011 Sep 23. PubMed PMID:
21949129; PubMed Central PMCID: PMC3220486.

44: Pollegioni P, Van der Linden G, Belisario A, Gras M, Anselmi N, Olimpieri I,
Luongo L, Santini A, Turco E, Scarascia Mugnozza G, Malvolti ME. Mechanisms
governing the responses to anthracnose pathogen in Juglans spp. J Biotechnol.
2012 Jun 30;159(4):251-64. doi: 10.1016/j.jbiotec.2011.08.020. Epub 2011 Aug 22.
PubMed PMID: 21884735.

45: Volontè F, Piubelli L, Pollegioni L. Optimizing HIV-1 protease production in
Escherichia coli as fusion protein. Microb Cell Fact. 2011 Jun 30;10:53. doi:
10.1186/1475-2859-10-53. PubMed PMID: 21718537; PubMed Central PMCID: PMC3141379.

46: Fossat P, Turpin FR, Sacchi S, Dulong J, Shi T, Rivet JM, Sweedler JV,
Pollegioni L, Millan MJ, Oliet SH, Mothet JP. Glial D-serine gates NMDA receptors
at excitatory synapses in prefrontal cortex. Cereb Cortex. 2012
Mar;22(3):595-606. doi: 10.1093/cercor/bhr130. Epub 2011 Jun 20. PubMed PMID:

47: Sacchi S, Cappelletti P, Giovannardi S, Pollegioni L. Evidence for the
interaction of D-amino acid oxidase with pLG72 in a glial cell line. Mol Cell
Neurosci. 2011 Sep;48(1):20-8. doi: 10.1016/j.mcn.2011.06.001. Epub 2011 Jun 12.
PubMed PMID: 21679769.

48: Pollegioni L, Schonbrunn E, Siehl D. Molecular basis of glyphosate
resistance-different approaches through protein engineering. FEBS J. 2011
Aug;278(16):2753-66. doi: 10.1111/j.1742-4658.2011.08214.x. Epub 2011 Jun 28.
Review. PubMed PMID: 21668647; PubMed Central PMCID: PMC3145815.

49: Vasylieva N, Barnych B, Meiller A, Maucler C, Pollegioni L, Lin JS, Barbier
D, Marinesco S. Covalent enzyme immobilization by poly(ethylene glycol)
diglycidyl ether (PEGDE) for microelectrode biosensor preparation. Biosens
Bioelectron. 2011 Jun 15;26(10):3993-4000. doi: 10.1016/j.bios.2011.03.012. Epub
2011 Apr 6. PubMed PMID: 21546239.

50: Frattini L, Rosini E, Pollegioni L, Pilone MS. Analyzing the D-amino acid
content in biological samples by engineered enzymes. J Chromatogr B Analyt
Technol Biomed Life Sci. 2011 Nov 1;879(29):3235-9. doi:
10.1016/j.jchromb.2011.02.036. Epub 2011 Mar 1. PubMed PMID: 21419721.

51: Pollegioni L, Molla G. New biotech applications from evolved D-amino acid
oxidases. Trends Biotechnol. 2011 Jun;29(6):276-83. doi:
10.1016/j.tibtech.2011.01.010. Epub 2011 Mar 10. Review. PubMed PMID: 21397351.

52: Rosini E, Molla G, Ghisla S, Pollegioni L. On the reaction of D-amino acid
oxidase with dioxygen: O2 diffusion pathways and enhancement of reactivity. FEBS
J. 2011 Feb;278(3):482-92. doi: 10.1111/j.1742-4658.2010.07969.x. Epub 2010 Dec
23. PubMed PMID: 21182588.

53: Caldinelli L, Molla G, Bracci L, Lelli B, Pileri S, Cappelletti P, Sacchi S,
Pollegioni L. Effect of ligand binding on human D-amino acid oxidase:
implications for the development of new drugs for schizophrenia treatment.
Protein Sci. 2010 Aug;19(8):1500-12. doi: 10.1002/pro.429. PubMed PMID: 20521334;
PubMed Central PMCID: PMC2923503.

54: Saam J, Rosini E, Molla G, Schulten K, Pollegioni L, Ghisla S. O2 reactivity
of flavoproteins: dynamic access of dioxygen to the active site and role of a H+
relay system in D-amino acid oxidase. J Biol Chem. 2010 Aug 6;285(32):24439-46.
doi: 10.1074/jbc.M110.131193. Epub 2010 May 24. PubMed PMID: 20498362; PubMed
Central PMCID: PMC2915680.

55: Volontè F, Pollegioni L, Molla G, Frattini L, Marinelli F, Piubelli L.
Production of recombinant cholesterol oxidase containing covalently bound FAD in
Escherichia coli. BMC Biotechnol. 2010 Apr 21;10:33. doi:
10.1186/1472-6750-10-33. PubMed PMID: 20409334; PubMed Central PMCID: PMC2890692.

56: Pollegioni L, Sacchi S. Metabolism of the neuromodulator D-serine. Cell Mol
Life Sci. 2010 Jul;67(14):2387-404. doi: 10.1007/s00018-010-0307-9. Epub 2010 Mar
2. Review. PubMed PMID: 20195697.

57: Pedotti M, Rosini E, Molla G, Moschetti T, Savino C, Vallone B, Pollegioni L.
Glyphosate resistance by engineering the flavoenzyme glycine oxidase. J Biol
Chem. 2009 Dec 25;284(52):36415-23. doi: 10.1074/jbc.M109.051631. Epub 2009 Oct
28. PubMed PMID: 19864430; PubMed Central PMCID: PMC2794757.

58: Pollegioni L. Cholesterol oxidase: a model flavoprotein oxidase and a
biotechnological tool. FEBS J. 2009 Dec;276(23):6825. doi:
10.1111/j.1742-4658.2009.07376.x. Epub 2009 Oct 16. PubMed PMID: 19843170.

59: Pollegioni L, Piubelli L, Molla G. Cholesterol oxidase: biotechnological
applications. FEBS J. 2009 Dec;276(23):6857-70. doi:
10.1111/j.1742-4658.2009.07379.x. Epub 2009 Oct 16. Review. PubMed PMID:

60: Caldinelli L, Pedotti M, Motteran L, Molla G, Pollegioni L. FAD binding in
glycine oxidase from Bacillus subtilis. Biochimie. 2009
Nov-Dec;91(11-12):1499-508. doi: 10.1016/j.biochi.2009.09.003. Epub 2009 Sep 12.
PubMed PMID: 19751796.

61: Rosini E, Pollegioni L, Ghisla S, Orru R, Molla G. Optimization of D-amino
acid oxidase for low substrate concentrations--towards a cancer enzyme therapy.
FEBS J. 2009 Sep;276(17):4921-32. doi: 10.1111/j.1742-4658.2009.07191.x. Epub
2009 Aug 4. PubMed PMID: 19694805.

62: Donini S, Ferrari M, Fedeli C, Faini M, Lamberto I, Marletta AS, Mellini L,
Panini M, Percudani R, Pollegioni L, Caldinelli L, Petrucco S, Peracchi A.
Recombinant production of eight human cytosolic aminotransferases and assessment
of their potential involvement in glyoxylate metabolism. Biochem J. 2009 Aug
13;422(2):265-72. doi: 10.1042/BJ20090748. PubMed PMID: 19545238.

63: Romano D, Molla G, Pollegioni L, Marinelli F. Optimization of human D-amino
acid oxidase expression in Escherichia coli. Protein Expr Purif. 2009
Nov;68(1):72-8. doi: 10.1016/j.pep.2009.05.013. Epub 2009 Jun 2. PubMed PMID:

64: Caldinelli L, Molla G, Sacchi S, Pilone MS, Pollegioni L. Relevance of weak
flavin binding in human D-amino acid oxidase. Protein Sci. 2009 Apr;18(4):801-10.
doi: 10.1002/pro.86. PubMed PMID: 19309736; PubMed Central PMCID: PMC2762592.

65: Pedotti M, Ghisla S, Motteran L, Molla G, Pollegioni L. Catalytic and redox
properties of glycine oxidase from Bacillus subtilis. Biochimie. 2009
May;91(5):604-12. doi: 10.1016/j.biochi.2009.02.007. Epub 2009 Feb 28. PubMed
PMID: 19254749.

66: Piubelli L, Pedotti M, Molla G, Feindler-Boeckh S, Ghisla S, Pilone MS,
Pollegioni L. On the oxygen reactivity of flavoprotein oxidases: an oxygen access
tunnel and gate in brevibacterium sterolicum cholesterol oxidase. J Biol Chem.
2008 Sep 5;283(36):24738-47. doi: 10.1074/jbc.M802321200. Epub 2008 Jul 9. PubMed
PMID: 18614534; PubMed Central PMCID: PMC3259834.

67: Rosini E, Molla G, Rossetti C, Pilone MS, Pollegioni L, Sacchi S. A biosensor
for all D-amino acids using evolved D-amino acid oxidase. J Biotechnol. 2008 Jul
31;135(4):377-84. doi: 10.1016/j.jbiotec.2008.06.001. Epub 2008 Jun 8. PubMed
PMID: 18588925.

68: Volontè F, Marinelli F, Gastaldo L, Sacchi S, Pilone MS, Pollegioni L, Molla
G. Optimization of glutaryl-7-aminocephalosporanic acid acylase expression in E.
coli. Protein Expr Purif. 2008 Oct;61(2):131-7. doi: 10.1016/j.pep.2008.05.010.
Epub 2008 May 28. PubMed PMID: 18586517.

69: Sacchi S, Bernasconi M, Martineau M, Mothet JP, Ruzzene M, Pilone MS,
Pollegioni L, Molla G. pLG72 modulates intracellular D-serine levels through its
interaction with D-amino acid oxidase: effect on schizophrenia susceptibility. J
Biol Chem. 2008 Aug 8;283(32):22244-56. doi: 10.1074/jbc.M709153200. Epub 2008
Jun 10. PubMed PMID: 18544534.

70: Pernot P, Mothet JP, Schuvailo O, Soldatkin A, Pollegioni L, Pilone M,
Adeline MT, Cespuglio R, Marinesco S. Characterization of a yeast D-amino acid
oxidase microbiosensor for D-serine detection in the central nervous system. Anal
Chem. 2008 Mar 1;80(5):1589-97. doi: 10.1021/ac702230w. Epub 2008 Jan 30. PubMed
PMID: 18229946.

71: Pollegioni L, Sacchi S, Caldinelli L, Boselli A, Pilone MS, Piubelli L, Molla
G. Engineering the properties of D-amino acid oxidases by a rational and a
directed evolution approach. Curr Protein Pept Sci. 2007 Dec;8(6):600-18. Review.
PubMed PMID: 18220846.

72: Caldinelli L, Iametti S, Barbiroli A, Fessas D, Bonomi F, Piubelli L, Molla
G, Pollegioni L. Relevance of the flavin binding to the stability and folding of
engineered cholesterol oxidase containing noncovalently bound FAD. Protein Sci.
2008 Mar;17(3):409-19. doi: 10.1110/ps.073137708. Epub 2008 Jan 24. PubMed PMID:
18218720; PubMed Central PMCID: PMC2248306.

73: Pollegioni L, Molla G, Sacchi S, Rosini E, Verga R, Pilone MS. Properties and
applications of microbial D-amino acid oxidases: current state and perspectives.
Appl Microbiol Biotechnol. 2008 Feb;78(1):1-16. Epub 2007 Dec 15. Review. PubMed
PMID: 18084756.

74: Kay CW, El Mkami H, Molla G, Pollegioni L, Ramsay RR. Characterization of the
covalently bound anionic flavin radical in monoamine oxidase a by electron
paramagnetic resonance. J Am Chem Soc. 2007 Dec 26;129(51):16091-7. Epub 2007 Nov
29. PubMed PMID: 18044898.

75: Boselli A, Rosini E, Marcone GL, Sacchi S, Motteran L, Pilone MS, Pollegioni
L, Molla G. Glycine oxidase from Bacillus subtilis: role of histidine 244 and
methionine 261. Biochimie. 2007 Nov;89(11):1372-80. Epub 2007 May 8. PubMed PMID:

76: Pollegioni L, Piubelli L, Sacchi S, Pilone MS, Molla G. Physiological
functions of D-amino acid oxidases: from yeast to humans. Cell Mol Life Sci. 2007
Jun;64(11):1373-94. Review. PubMed PMID: 17396222.

77: Boselli A, Piubelli L, Molla G, Pilone MS, Pollegioni L, Sacchi S.
Investigating the role of active site residues of Rhodotorula gracilis D-amino
acid oxidase on its substrate specificity. Biochimie. 2007 Mar;89(3):360-8. Epub
2006 Nov 27. PubMed PMID: 17145127.

78: Lim L, Molla G, Guinn N, Ghisla S, Pollegioni L, Vrielink A. Structural and
kinetic analyses of the H121A mutant of cholesterol oxidase. Biochem J. 2006 Nov
15;400(1):13-22. PubMed PMID: 16856877; PubMed Central PMCID: PMC1635447.

79: Panatier A, Theodosis DT, Mothet JP, Touquet B, Pollegioni L, Poulain DA,
Oliet SH. Glia-derived D-serine controls NMDA receptor activity and synaptic
memory. Cell. 2006 May 19;125(4):775-84. PubMed PMID: 16713567.

80: Caldinelli L, Molla G, Pilone MS, Pollegioni L. Tryptophan 243 affects
interprotein contacts, cofactor binding and stability in D-amino acid oxidase
from Rhodotorula gracilis. FEBS J. 2006 Feb;273(3):504-12. PubMed PMID: 16420474.

81: Pollegioni L, Lorenzi S, Rosini E, Marcone GL, Molla G, Verga R, Cabri W,
Pilone MS. Evolution of an acylase active on cephalosporin C. Protein Sci. 2005
Dec;14(12):3064-76. Epub 2005 Oct 31. PubMed PMID: 16260759; PubMed Central
PMCID: PMC2253238.

82: Molla G, Bernasconi M, Sacchi S, Pilone MS, Pollegioni L. Expression in
Escherichia coli and in vitro refolding of the human protein pLG72. Protein Expr
Purif. 2006 Mar;46(1):150-5. Epub 2005 Sep 7. PubMed PMID: 16183301.

83: Caldinelli L, Iametti S, Barbiroli A, Bonomi F, Fessas D, Molla G, Pilone MS,
Pollegioni L. Dissecting the structural determinants of the stability of
cholesterol oxidase containing covalently bound flavin. J Biol Chem. 2005 Jun
17;280(24):22572-81. Epub 2005 Apr 7. PubMed PMID: 15817448.

84: Mothet JP, Pollegioni L, Ouanounou G, Martineau M, Fossier P, Baux G.
Glutamate receptor activation triggers a calcium-dependent and SNARE
protein-dependent release of the gliotransmitter D-serine. Proc Natl Acad Sci U S
A. 2005 Apr 12;102(15):5606-11. Epub 2005 Mar 30. PubMed PMID: 15800046; PubMed
Central PMCID: PMC556243.

85: Boselli A, Piubelli L, Molla G, Sacchi S, Pilone MS, Ghisla S, Pollegioni L.
On the mechanism of Rhodotorula gracilis D-amino acid oxidase: role of the active
site serine 335. Biochim Biophys Acta. 2004 Oct 1;1702(1):19-32. PubMed PMID:

86: Sacchi S, Rosini E, Molla G, Pilone MS, Pollegioni L. Modulating D-amino acid
oxidase substrate specificity: production of an enzyme for analytical
determination of all D-amino acids by directed evolution. Protein Eng Des Sel.
2004 Jun;17(6):517-25. Epub 2004 Aug 13. PubMed PMID: 15310841.

87: Mörtl M, Diederichs K, Welte W, Molla G, Motteran L, Andriolo G, Pilone MS,
Pollegioni L. Structure-function correlation in glycine oxidase from Bacillus
subtilis. J Biol Chem. 2004 Jul 9;279(28):29718-27. Epub 2004 Apr 22. PubMed
PMID: 15105420.

88: Caldinelli L, Iametti S, Barbiroli A, Bonomi F, Piubelli L, Ferranti P,
Picariello G, Pilone MS, Pollegioni L. Unfolding intermediate in the peroxisomal
flavoprotein D-amino acid oxidase. J Biol Chem. 2004 Jul 2;279(27):28426-34. Epub
2004 Apr 21. PubMed PMID: 15102841.

89: Pollegioni L, Caldinelli L, Molla G, Sacchi S, Pilone MS. Catalytic
properties of D-amino acid oxidase in cephalosporin C bioconversion: a comparison
between proteins from different sources. Biotechnol Prog. 2004
Mar-Apr;20(2):467-73. PubMed PMID: 15058991.

90: Piubelli L, Molla G, Caldinelli L, Pilone MS, Pollegioni L. Dissection of the
structural determinants involved in formation of the dimeric form of D-amino acid
oxidase from Rhodotorula gracilis: role of the size of the betaF5-betaF6 loop.
Protein Eng. 2003 Dec;16(12):1063-9. PubMed PMID: 14983088.

91: Molla G, Motteran L, Piubelli L, Pilone MS, Pollegioni L. Regulation of
D-amino acid oxidase expression in the yeast Rhodotorula gracilis. Yeast. 2003
Sep;20(12):1061-9. PubMed PMID: 12961754.

92: Pollegioni L, Iametti S, Fessas D, Caldinelli L, Piubelli L, Barbiroli A,
Pilone MS, Bonomi F. Contribution of the dimeric state to the thermal stability
of the flavoprotein D-amino acid oxidase. Protein Sci. 2003 May;12(5):1018-29.
PubMed PMID: 12717024; PubMed Central PMCID: PMC2323872.

93: Molla G, Motteran L, Job V, Pilone MS, Pollegioni L. Kinetic mechanisms of
glycine oxidase from Bacillus subtilis. Eur J Biochem. 2003 Apr;270(7):1474-82.
PubMed PMID: 12654003.

94: Pollegioni L, Diederichs K, Molla G, Umhau S, Welte W, Ghisla S, Pilone MS.
Yeast D-amino acid oxidase: structural basis of its catalytic properties. J Mol
Biol. 2002 Nov 29;324(3):535-46. PubMed PMID: 12445787.

95: Boselli A, Sacchi S, Job V, Pilone MS, Pollegioni L. Role of tyrosine 238 in
the active site of Rhodotorula gracilis D-amino acid oxidase. A site-directed
mutagenesis study. Eur J Biochem. 2002 Oct;269(19):4762-71. PubMed PMID:

96: Piubelli L, Caldinelli L, Molla G, Pilone MS, Pollegioni L. Conversion of the
dimeric D-amino acid oxidase from Rhodotorula gracilis to a monomeric form. A
rational mutagenesis approach. FEBS Lett. 2002 Aug 28;526(1-3):43-8. PubMed PMID:

97: Sacchi S, Lorenzi S, Molla G, Pilone MS, Rossetti C, Pollegioni L.
Engineering the substrate specificity of D-amino-acid oxidase. J Biol Chem. 2002
Jul 26;277(30):27510-6. Epub 2002 May 20. PubMed PMID: 12021281.

98: Ardini E, Sporchia B, Pollegioni L, Modugno M, Ghirelli C, Castiglioni F,
Tagliabue E, Ménard S. Identification of a novel function for 67-kDa laminin
receptor: increase in laminin degradation rate and release of motility fragments.
Cancer Res. 2002 Mar 1;62(5):1321-5. PubMed PMID: 11888899.

99: Job V, Molla G, Pilone MS, Pollegioni L. Overexpression of a recombinant
wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli.
Eur J Biochem. 2002 Mar;269(5):1456-63. PubMed PMID: 11874460.

100: Vigetti D, Pollegioni L, Monetti C, Prati M, Bernardini G, Gornati R.
Property comparison of recombinant amphibian and mammalian allantoicases. FEBS
Lett. 2002 Feb 13;512(1-3):323-8. PubMed PMID: 11852104.

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