Research

Over the past decade, our laboratory has been focused on advancing research in the field of membrane proteins, specifically on those involved in the reuptake of neurotransmitters, nutrients, and divalent cations (such as SLC1, SLC6, SLC11, and SLC15) in the brain and the gut.

Our multifaceted approach integrates electrophysiology, molecular biology, and cutting-edge optical techniques to investigate the intricate structure-function relationships of these proteins. 

Techniques
The research activity involves the use of several state-of the art methodologies, including heterologous expression in oocytes and cell lines, patch-clamp, voltage-clamp, confocal microscopy, fluorescence resonance energy transfer, caged–compounds photolysis, fluorescent ion indicators, green fluorescent protein constructs, site-directed mutagenesis, immunochemistry (SOC, western Blot and immunohistochemistry) as well as other techniques in molecular biology and cell culture. Quantitative biophysical analysis of the results will aim to obtain detailed description of transporter functioning and regulation.

The Laboratory of cellular and molecular Physiology is part of the Department of Biotechnology and Life Sciences (DBSV) of the University of Insubria.  The DBSV building includes all facilities necessary for modern biological research at the cellular and molecular level. The DBSV hosts other research groups in related areas, a Biomedical Library and support services.

Please, visit the web site of the DBSV, click here.

Dipartimento di Biotecnologie e Scienze della Vita